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To a test tube containing 25 mg of Zein add 1 mL of nitric acid. Agitate vigorously: the solution becomes light yellow. Further addition of about 10 mL of 6 N ammonium hydroxide produces an orange color.
It is insoluble in water, but 1 g of it in 10 mL of a mixture of isopropyl alcohol and water (85:15), at a temperature of about 37
, yields a clear to cloudy solution.
61
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The total bacterial count does not exceed 1000 cfu per g, and the tests for Salmonella species and Escherichia coli are negative.
731—
Dry it at 105 for 2 hours: it loses not more than 8.0% of its weight.
281:
not more than 2.0%.
231:
0.002%.
467:
meets the requirements.
461: the nitrogen content is not less than 13.1% and not more than 17.0%, on the dried basis.
467:
meets the requirements.