Acid Stage
Place 1000 mL of 0.1 N hydrochloric acid in the vessel, and assemble the apparatus. Allow the medium to equilibrate to a temperature of 37 ± 0.5
. Place 1 tablet or 1 capsule in the apparatus, cover the vessel, and operate the apparatus for 2 hours at the rate specified in the monograph. After 2 hours of operation in 0.1 N hydrochloric acid, withdraw an aliquot of the fluid, and proceed immediately as directed under
Buffer Stage.
Perform an analysis of the aliquot using the Procedure specified in the test for Drug release in the individual monograph.
Unless otherwise specified in the individual monograph, the requirements of this portion of the test are met if the quantities, based on the percentage of the labeled content, of active ingredient dissolved from the units tested conform to
Acceptance Table 2 under
Method A. Continue testing through all levels unless the results of both acid and buffer stages conform at an earlier level.
Buffer Stage
[NOTEFor this stage of the procedure, use buffer that previously has been equilibrated to a temperature of 37 ± 0.5
.
] Drain the acid from the vessel, and add to the vessel 1000 mL of pH 6.8 phosphate buffer, prepared by mixing 0.1 N hydrochloric acid with 0.20 M tribasic sodium phosphate (3:1) and adjusting, if necessary, with 2 N hydrochloric acid or 2 N sodium hydroxide to a pH of 6.8 ± 0.05.
[NOTEThis may be accomplished also by removing from the apparatus the vessel containing the acid and replacing it with another vessel containing the buffer and transferring the dosage unit to the vessel containing the buffer.
] Continue to operate the apparatus for 45 minutes, or for the time specified in the individual monograph. At the end of the time period, withdraw an aliquot of the fluid, and perform the analysis using the
Procedure specified in the test for
Drug release in the individual monograph. The test may be concluded in a shorter time period than that specified for the
Buffer Stage if the requirement for minimum amount dissolved is met at an earlier time.